Construct: shRNA TRCN0000001943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014911.x-629s1c1
DNA Barcode:
CCAGGTGTGCAAGAGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AAK1 (22848)
Gene Description:
AP2 associated kinase 1
Transcript:
RefSeq NM_014911.2 (NON-CURRENT)
Match location:
Position 629 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGTGTGCAAGAGAGAAAT
Hairpin Sequence:
5'-CCGG-CCAGGTGTGCAAGAGAGAAAT-CTCGAG-ATTTCTCTCTTGCACACCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGTGTGCAAGAGAGAAATCTCGAGATTTCTCTCTTGCACACCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGTGTGCAAGAGAGAAATCTCGAGATTTCTCTCTTGCACACCTGG-3'

Other clones with same target sequence:

(none)