Construct: shRNA TRCN0000001978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170754.x-440s1c1
DNA Barcode:
CCACCAAATCTCTGAACCACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNS2 (23371)
Gene Description:
tensin 2
Transcript:
RefSeq NM_170754.2 (NON-CURRENT)
Match location:
Position 440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCAAATCTCTGAACCACT
Hairpin Sequence:
5'-CCGG-CCACCAAATCTCTGAACCACT-CTCGAG-AGTGGTTCAGAGATTTGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCAAATCTCTGAACCACTCTCGAGAGTGGTTCAGAGATTTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCAAATCTCTGAACCACTCTCGAGAGTGGTTCAGAGATTTGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000272947