Construct: shRNA TRCN0000001999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002609.x-4435s1c1
DNA Barcode:
CCCTCAGTCTTAATCCATCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDGFRB (5159)
Gene Description:
platelet derived growth factor receptor beta
Transcript:
RefSeq NM_002609.2 (NON-CURRENT)
Match location:
Position 4435 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000001999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGTCTTAATCCATCCA
Hairpin Sequence:
5'-CCGG-CCCTCAGTCTTAATCCATCCA-CTCGAG-TGGATGGATTAAGACTGAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGTCTTAATCCATCCACTCGAGTGGATGGATTAAGACTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGTCTTAATCCATCCACTCGAGTGGATGGATTAAGACTGAGGG-3'

Other clones with same target sequence:

(none)