Construct: shRNA TRCN0000002005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002731.x-150s1c1
DNA Barcode:
ACTCAGAATAATGCCGGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKACB (5567)
Gene Description:
protein kinase cAMP-activated catalytic subunit beta
Transcript:
RefSeq NM_002731.1 (NON-CURRENT)
Match location:
Position 150 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCAGAATAATGCCGGACTT
Hairpin Sequence:
5'-CCGG-ACTCAGAATAATGCCGGACTT-CTCGAG-AAGTCCGGCATTATTCTGAGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCAGAATAATGCCGGACTTCTCGAGAAGTCCGGCATTATTCTGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCAGAATAATGCCGGACTTCTCGAGAAGTCCGGCATTATTCTGAGT-3'

Other clones with same target sequence:

(none)