Construct: shRNA TRCN0000002039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005781.x-4413s1c1
DNA Barcode:
TGCTTCCTCTTCCACCCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNK2 (10188)
Gene Description:
tyrosine kinase non receptor 2
Transcript:
RefSeq NM_005781.2 (NON-CURRENT)
Match location:
Position 4413 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTTCCTCTTCCACCCAATT
Hairpin Sequence:
5'-CCGG-TGCTTCCTCTTCCACCCAATT-CTCGAG-AATTGGGTGGAAGAGGAAGCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTTCCTCTTCCACCCAATTCTCGAGAATTGGGTGGAAGAGGAAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTTCCTCTTCCACCCAATTCTCGAGAATTGGGTGGAAGAGGAAGCA-3'

Other clones with same target sequence:

(none)