Construct: shRNA TRCN0000002074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024717.x-528s1c1
DNA Barcode:
CCTTTCTGGATCTGACACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCTP1 (79772)
Gene Description:
multiple C2 and transmembrane domain containing 1
Transcript:
RefSeq NM_024717.2 (NON-CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTGGATCTGACACAAT
Hairpin Sequence:
5'-CCGG-CCTTTCTGGATCTGACACAAT-CTCGAG-ATTGTGTCAGATCCAGAAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTGGATCTGACACAATCTCGAGATTGTGTCAGATCCAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTGGATCTGACACAATCTCGAGATTGTGTCAGATCCAGAAAGG-3'

Other clones with same target sequence:

(none)