Construct: shRNA TRCN0000002081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030906.x-3035s1c1
DNA Barcode:
CTTGCCATTAACTTGCTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK33 (65975)
Gene Description:
serine/threonine kinase 33
Transcript:
RefSeq NM_030906.1 (NON-CURRENT)
Match location:
Position 3035 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGCCATTAACTTGCTGCTA
Hairpin Sequence:
5'-CCGG-CTTGCCATTAACTTGCTGCTA-CTCGAG-TAGCAGCAAGTTAATGGCAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGCCATTAACTTGCTGCTACTCGAGTAGCAGCAAGTTAATGGCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGCCATTAACTTGCTGCTACTCGAGTAGCAGCAAGTTAATGGCAAG-3'

Other clones with same target sequence:

(none)