Construct: shRNA TRCN0000002143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004714.x-1353s1c1
DNA Barcode:
GAAGGACGAAAGAACTCAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYRK1B (9149)
Gene Description:
dual specificity tyrosine phosphorylation regulated kinase 1B
Transcript:
RefSeq NM_004714.1 (NON-CURRENT)
Match location:
Position 1353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGACGAAAGAACTCAGGA
Hairpin Sequence:
5'-CCGG-GAAGGACGAAAGAACTCAGGA-CTCGAG-TCCTGAGTTCTTTCGTCCTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGACGAAAGAACTCAGGACTCGAGTCCTGAGTTCTTTCGTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGACGAAAGAACTCAGGACTCGAGTCCTGAGTTCTTTCGTCCTTC-3'

Other clones with same target sequence:

(none)