Construct: shRNA TRCN0000002153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004815.x-801s1c1
DNA Barcode:
GCTGGCTTTGTCATATGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP29 (9411)
Gene Description:
Rho GTPase activating protein 29
Transcript:
RefSeq NM_004815.1 (NON-CURRENT)
Match location:
Position 801 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCTTTGTCATATGCTAA
Hairpin Sequence:
5'-CCGG-GCTGGCTTTGTCATATGCTAA-CTCGAG-TTAGCATATGACAAAGCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCTTTGTCATATGCTAACTCGAGTTAGCATATGACAAAGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCTTTGTCATATGCTAACTCGAGTTAGCATATGACAAAGCCAGC-3'

Other clones with same target sequence:

(none)