Construct: shRNA TRCN0000002163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005406.x-3241s1c1
DNA Barcode:
GCCAGCAAAGAGAGTGATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROCK1 (6093)
Gene Description:
Rho associated coiled-coil containing protein kinase 1
Transcript:
RefSeq NM_005406.1 (NON-CURRENT)
Match location:
Position 3241 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCAAAGAGAGTGATATT
Hairpin Sequence:
5'-CCGG-GCCAGCAAAGAGAGTGATATT-CTCGAG-AATATCACTCTCTTTGCTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCAAAGAGAGTGATATTCTCGAGAATATCACTCTCTTTGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCAAAGAGAGTGATATTCTCGAGAATATCACTCTCTTTGCTGGC-3'

Other clones with same target sequence:

(none)