Construct: shRNA TRCN0000002227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002576.x-2127s1c1
DNA Barcode:
CTTCTCCCATTTCCTGATCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK1 (5058)
Gene Description:
p21 (RAC1) activated kinase 1
Transcript:
RefSeq NM_002576.2 (NON-CURRENT)
Match location:
Position 2127 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTCCCATTTCCTGATCTA
Hairpin Sequence:
5'-CCGG-CTTCTCCCATTTCCTGATCTA-CTCGAG-TAGATCAGGAAATGGGAGAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTCCCATTTCCTGATCTACTCGAGTAGATCAGGAAATGGGAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTCCCATTTCCTGATCTACTCGAGTAGATCAGGAAATGGGAGAAG-3'

Other clones with same target sequence:

(none)