Construct: shRNA TRCN0000002227
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002576.x-2127s1c1
- DNA Barcode:
- CTTCTCCCATTTCCTGATCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK1 (5058)
- Gene Description:
- p21 (RAC1) activated kinase 1
- Transcript:
- RefSeq NM_002576.2 (NON-CURRENT)
- Match location:
- Position 2127 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000002227)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTTCTCCCATTTCCTGATCTA
- Hairpin Sequence:
-
5'-CCGG-CTTCTCCCATTTCCTGATCTA-CTCGAG-TAGATCAGGAAATGGGAGAAG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTTCTCCCATTTCCTGATCTACTCGAGTAGATCAGGAAATGGGAGAAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTTCTCCCATTTCCTGATCTACTCGAGTAGATCAGGAAATGGGAGAAG-3'
Other clones with same target sequence:
(none)
