Construct: shRNA TRCN0000002252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006648.x-4029s1c1
DNA Barcode:
CCAAGATTCAGCGCCCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNK2 (65268)
Gene Description:
WNK lysine deficient protein kinase 2
Transcript:
RefSeq NM_006648.3 (CURRENT)
Match location:
Position 4029 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGATTCAGCGCCCTATAA
Hairpin Sequence:
5'-CCGG-CCAAGATTCAGCGCCCTATAA-CTCGAG-TTATAGGGCGCTGAATCTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGATTCAGCGCCCTATAACTCGAGTTATAGGGCGCTGAATCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGATTCAGCGCCCTATAACTCGAGTTATAGGGCGCTGAATCTTGG-3'

Other clones with same target sequence:

(none)