Construct: shRNA TRCN0000002267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014586.x-1764s1c1
DNA Barcode:
AGGCTCGCTTATGACACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HUNK (30811)
Gene Description:
hormonally up-regulated Neu-associated kinase
Transcript:
RefSeq NM_014586.1 (NON-CURRENT)
Match location:
Position 1764 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCTCGCTTATGACACAGAT
Hairpin Sequence:
5'-CCGG-AGGCTCGCTTATGACACAGAT-CTCGAG-ATCTGTGTCATAAGCGAGCCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCTCGCTTATGACACAGATCTCGAGATCTGTGTCATAAGCGAGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCTCGCTTATGACACAGATCTCGAGATCTGTGTCATAAGCGAGCCT-3'

Other clones with same target sequence:

(none)