Construct: shRNA TRCN0000002285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032960.x-2982s1c1
DNA Barcode:
CCAGCACTCGATTGTTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK2 (9261)
Gene Description:
MAPK activated protein kinase 2
Transcript:
RefSeq NM_032960.2 (NON-CURRENT)
Match location:
Position 2982 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCACTCGATTGTTGTAAA
Hairpin Sequence:
5'-CCGG-CCAGCACTCGATTGTTGTAAA-CTCGAG-TTTACAACAATCGAGTGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCACTCGATTGTTGTAAACTCGAGTTTACAACAATCGAGTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCACTCGATTGTTGTAAACTCGAGTTTACAACAATCGAGTGCTGG-3'

Other clones with same target sequence:

(none)