Construct: shRNA TRCN0000002301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153499.x-5390s1c1
DNA Barcode:
CGACCCTTTCTACTATGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMKK2 (10645)
Gene Description:
calcium/calmodulin dependent protein kinase kinase 2
Transcript:
RefSeq NM_153499.2 (CURRENT)
Match location:
Position 5390 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCCTTTCTACTATGCATT
Hairpin Sequence:
5'-CCGG-CGACCCTTTCTACTATGCATT-CTCGAG-AATGCATAGTAGAAAGGGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCCTTTCTACTATGCATTCTCGAGAATGCATAGTAGAAAGGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCCTTTCTACTATGCATTCTCGAGAATGCATAGTAGAAAGGGTCG-3'

Other clones with same target sequence:

(none)