Construct: shRNA TRCN0000002318

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002611.x-1929s1c1
DNA Barcode:
ACCTTGTTAGACCGAGAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDK2 (5164)
Gene Description:
pyruvate dehydrogenase kinase 2
Transcript:
RefSeq NM_002611.3 (NON-CURRENT)
Match location:
Position 1929 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002318)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTTGTTAGACCGAGAGCTT
Hairpin Sequence:
5'-CCGG-ACCTTGTTAGACCGAGAGCTT-CTCGAG-AAGCTCTCGGTCTAACAAGGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTTGTTAGACCGAGAGCTTCTCGAGAAGCTCTCGGTCTAACAAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTTGTTAGACCGAGAGCTTCTCGAGAAGCTCTCGGTCTAACAAGGT-3'

Other clones with same target sequence:

(none)