Construct: shRNA TRCN0000002443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002480.x-1772s1c1
DNA Barcode:
GCACTACTACAAAGATTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R12A (4659)
Gene Description:
protein phosphatase 1 regulatory subunit 12A
Transcript:
RefSeq NM_002480.1 (NON-CURRENT)
Match location:
Position 1772 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTACTACAAAGATTACAA
Hairpin Sequence:
5'-CCGG-GCACTACTACAAAGATTACAA-CTCGAG-TTGTAATCTTTGTAGTAGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTACTACAAAGATTACAACTCGAGTTGTAATCTTTGTAGTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTACTACAAAGATTACAACTCGAGTTGTAATCTTTGTAGTAGTGC-3'

Other clones with same target sequence:

(none)