Construct: shRNA TRCN0000002458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002709.x-1216s1c1
DNA Barcode:
ACAGCTAATCCGCCGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1CB (5500)
Gene Description:
protein phosphatase 1 catalytic subunit beta
Transcript:
RefSeq NM_002709.1 (NON-CURRENT)
Match location:
Position 1216 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGCTAATCCGCCGAAGAAA
Hairpin Sequence:
5'-CCGG-ACAGCTAATCCGCCGAAGAAA-CTCGAG-TTTCTTCGGCGGATTAGCTGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGCTAATCCGCCGAAGAAACTCGAGTTTCTTCGGCGGATTAGCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGCTAATCCGCCGAAGAAACTCGAGTTTCTTCGGCGGATTAGCTGT-3'

Other clones with same target sequence:

(none)