Construct: shRNA TRCN0000002475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002713.x-2306s1c1
DNA Barcode:
CATAGGAGACAAAGTTAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R8 (5511)
Gene Description:
protein phosphatase 1 regulatory subunit 8
Transcript:
RefSeq NM_002713.2 (NON-CURRENT)
Match location:
Position 2306 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGGAGACAAAGTTAGGAA
Hairpin Sequence:
5'-CCGG-CATAGGAGACAAAGTTAGGAA-CTCGAG-TTCCTAACTTTGTCTCCTATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGGAGACAAAGTTAGGAACTCGAGTTCCTAACTTTGTCTCCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGGAGACAAAGTTAGGAACTCGAGTTCCTAACTTTGTCTCCTATG-3'

Other clones with same target sequence:

(none)