Construct: shRNA TRCN0000002497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002719.x-2823s1c1
DNA Barcode:
CGGTCTTCACATGCTGCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R5C (5527)
Gene Description:
protein phosphatase 2 regulatory subunit B'gamma
Transcript:
RefSeq NM_002719.2 (NON-CURRENT)
Match location:
Position 2823 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCTTCACATGCTGCTATA
Hairpin Sequence:
5'-CCGG-CGGTCTTCACATGCTGCTATA-CTCGAG-TATAGCAGCATGTGAAGACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCTTCACATGCTGCTATACTCGAGTATAGCAGCATGTGAAGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCTTCACATGCTGCTATACTCGAGTATAGCAGCATGTGAAGACCG-3'

Other clones with same target sequence:

(none)