Construct: shRNA TRCN0000002560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006246.x-2461s1c1
DNA Barcode:
CCTCTGGATAACTTGACTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R5E (5529)
Gene Description:
protein phosphatase 2 regulatory subunit B'epsilon
Transcript:
RefSeq NM_006246.2 (NON-CURRENT)
Match location:
Position 2461 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGGATAACTTGACTGTA
Hairpin Sequence:
5'-CCGG-CCTCTGGATAACTTGACTGTA-CTCGAG-TACAGTCAAGTTATCCAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGGATAACTTGACTGTACTCGAGTACAGTCAAGTTATCCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGGATAACTTGACTGTACTCGAGTACAGTCAAGTTATCCAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000272842