Construct: shRNA TRCN0000002570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021003.x-2115s1c1
DNA Barcode:
CCTTCGTTGTTTCCATGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPM1A (5494)
Gene Description:
protein phosphatase, Mg2+/Mn2+ dependent 1A
Transcript:
RefSeq NM_021003.2 (NON-CURRENT)
Match location:
Position 2115 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGTTGTTTCCATGAGTA
Hairpin Sequence:
5'-CCGG-CCTTCGTTGTTTCCATGAGTA-CTCGAG-TACTCATGGAAACAACGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGTTGTTTCCATGAGTACTCGAGTACTCATGGAAACAACGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGTTGTTTCCATGAGTACTCGAGTACTCATGGAAACAACGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000297347