Construct: shRNA TRCN0000002571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021003.x-507s1c1
DNA Barcode:
GTGGTCATTCTTTGCTGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPM1A (5494)
Gene Description:
protein phosphatase, Mg2+/Mn2+ dependent 1A
Transcript:
RefSeq NM_021003.2 (NON-CURRENT)
Match location:
Position 507 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTCATTCTTTGCTGTGTA
Hairpin Sequence:
5'-CCGG-GTGGTCATTCTTTGCTGTGTA-CTCGAG-TACACAGCAAAGAATGACCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTCATTCTTTGCTGTGTACTCGAGTACACAGCAAAGAATGACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTCATTCTTTGCTGTGTACTCGAGTACACAGCAAAGAATGACCAC-3'

Other clones with same target sequence:

  1. TRCN0000280109