Construct: shRNA TRCN0000002622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020386.x-307s1c1
DNA Barcode:
CCTGTACTTGGGTGATGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLAAT1 (57110)
Gene Description:
phospholipase A and acyltransferase 1
Transcript:
RefSeq NM_020386.1 (NON-CURRENT)
Match location:
Position 307 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTACTTGGGTGATGGTTA
Hairpin Sequence:
5'-CCGG-CCTGTACTTGGGTGATGGTTA-CTCGAG-TAACCATCACCCAAGTACAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTACTTGGGTGATGGTTACTCGAGTAACCATCACCCAAGTACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTACTTGGGTGATGGTTACTCGAGTAACCATCACCCAAGTACAGG-3'

Other clones with same target sequence:

(none)