Construct: shRNA TRCN0000002625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022485.x-1247s1c1
DNA Barcode:
GAAGCATATTACCTCCGAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTMR14 (64419)
Gene Description:
myotubularin related protein 14
Transcript:
RefSeq NM_022485.2 (NON-CURRENT)
Match location:
Position 1247 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCATATTACCTCCGAGGA
Hairpin Sequence:
5'-CCGG-GAAGCATATTACCTCCGAGGA-CTCGAG-TCCTCGGAGGTAATATGCTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCATATTACCTCCGAGGACTCGAGTCCTCGGAGGTAATATGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCATATTACCTCCGAGGACTCGAGTCCTCGGAGGTAATATGCTTC-3'

Other clones with same target sequence:

(none)