Construct: shRNA TRCN0000002653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030768.x-1147s1c1
DNA Barcode:
CTTCATCTTGTCCTGTCTCGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ILKAP (80895)
Gene Description:
ILK associated serine/threonine phosphatase
Transcript:
RefSeq NM_030768.2 (NON-CURRENT)
Match location:
Position 1147 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATCTTGTCCTGTCTCGA
Hairpin Sequence:
5'-CCGG-CTTCATCTTGTCCTGTCTCGA-CTCGAG-TCGAGACAGGACAAGATGAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATCTTGTCCTGTCTCGACTCGAGTCGAGACAGGACAAGATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATCTTGTCCTGTCTCGACTCGAGTCGAGACAGGACAAGATGAAG-3'

Other clones with same target sequence:

(none)