Construct: shRNA TRCN0000002682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080792.x-1119s1c1
DNA Barcode:
CACTGGATCTAATGAACGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIRPA (140885)
Gene Description:
signal regulatory protein alpha
Transcript:
RefSeq NM_080792.1 (NON-CURRENT)
Match location:
Position 1119 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGGATCTAATGAACGGAA
Hairpin Sequence:
5'-CCGG-CACTGGATCTAATGAACGGAA-CTCGAG-TTCCGTTCATTAGATCCAGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGGATCTAATGAACGGAACTCGAGTTCCGTTCATTAGATCCAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGGATCTAATGAACGGAACTCGAGTTCCGTTCATTAGATCCAGTG-3'

Other clones with same target sequence:

  1. TRCN0000342545