Construct: shRNA TRCN0000002691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080876.x-529s1c1
DNA Barcode:
CTATATTGGATCTGCCTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUSP19 (142679)
Gene Description:
dual specificity phosphatase 19
Transcript:
RefSeq NM_080876.2 (NON-CURRENT)
Match location:
Position 529 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATATTGGATCTGCCTGAAA
Hairpin Sequence:
5'-CCGG-CTATATTGGATCTGCCTGAAA-CTCGAG-TTTCAGGCAGATCCAATATAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATATTGGATCTGCCTGAAACTCGAGTTTCAGGCAGATCCAATATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATATTGGATCTGCCTGAAACTCGAGTTTCAGGCAGATCCAATATAG-3'

Other clones with same target sequence:

(none)