Construct: shRNA TRCN0000002790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002830.x-3240s1c1
DNA Barcode:
CCCTGATGATTCGAGTGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN4 (5775)
Gene Description:
protein tyrosine phosphatase non-receptor type 4
Transcript:
RefSeq NM_002830.2 (NON-CURRENT)
Match location:
Position 3240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGATGATTCGAGTGACTT
Hairpin Sequence:
5'-CCGG-CCCTGATGATTCGAGTGACTT-CTCGAG-AAGTCACTCGAATCATCAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGATGATTCGAGTGACTTCTCGAGAAGTCACTCGAATCATCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGATGATTCGAGTGACTTCTCGAGAAGTCACTCGAATCATCAGGG-3'

Other clones with same target sequence:

(none)