Construct: shRNA TRCN0000002828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002833.x-2517s1c1
DNA Barcode:
GCTATCAAACTGTGCCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN9 (5780)
Gene Description:
protein tyrosine phosphatase non-receptor type 9
Transcript:
RefSeq NM_002833.2 (NON-CURRENT)
Match location:
Position 2517 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCAAACTGTGCCTTATT
Hairpin Sequence:
5'-CCGG-GCTATCAAACTGTGCCTTATT-CTCGAG-AATAAGGCACAGTTTGATAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCAAACTGTGCCTTATTCTCGAGAATAAGGCACAGTTTGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCAAACTGTGCCTTATTCTCGAGAATAAGGCACAGTTTGATAGC-3'

Other clones with same target sequence:

(none)