Construct: shRNA TRCN0000002830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002835.x-245s1c1
DNA Barcode:
CAGCCGAGTTAAATTGACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN12 (5782)
Gene Description:
protein tyrosine phosphatase non-receptor type 12
Transcript:
RefSeq NM_002835.2 (NON-CURRENT)
Match location:
Position 245 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCGAGTTAAATTGACATT
Hairpin Sequence:
5'-CCGG-CAGCCGAGTTAAATTGACATT-CTCGAG-AATGTCAATTTAACTCGGCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCGAGTTAAATTGACATTCTCGAGAATGTCAATTTAACTCGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCGAGTTAAATTGACATTCTCGAGAATGTCAATTTAACTCGGCTG-3'

Other clones with same target sequence:

(none)