Construct: shRNA TRCN0000002874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002844.x-941s1c1
DNA Barcode:
GCCCAGACTAAGAACATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRK (5796)
Gene Description:
protein tyrosine phosphatase receptor type K
Transcript:
RefSeq NM_002844.2 (NON-CURRENT)
Match location:
Position 941 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGACTAAGAACATCAAT
Hairpin Sequence:
5'-CCGG-GCCCAGACTAAGAACATCAAT-CTCGAG-ATTGATGTTCTTAGTCTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGACTAAGAACATCAATCTCGAGATTGATGTTCTTAGTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGACTAAGAACATCAATCTCGAGATTGATGTTCTTAGTCTGGGC-3'

Other clones with same target sequence:

(none)