Construct: shRNA TRCN0000002882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002845.x-4874s1c1
DNA Barcode:
GAGTCGTCGTTTCTGTCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRM (5797)
Gene Description:
protein tyrosine phosphatase receptor type M
Transcript:
RefSeq NM_002845.2 (NON-CURRENT)
Match location:
Position 4874 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCGTCGTTTCTGTCAGAT
Hairpin Sequence:
5'-CCGG-GAGTCGTCGTTTCTGTCAGAT-CTCGAG-ATCTGACAGAAACGACGACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCGTCGTTTCTGTCAGATCTCGAGATCTGACAGAAACGACGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCGTCGTTTCTGTCAGATCTCGAGATCTGACAGAAACGACGACTC-3'

Other clones with same target sequence:

(none)