Construct: shRNA TRCN0000002885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002846.x-3266s1c1
DNA Barcode:
GAAAGGACATGGGTAGCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRN (5798)
Gene Description:
protein tyrosine phosphatase receptor type N
Transcript:
RefSeq NM_002846.2 (NON-CURRENT)
Match location:
Position 3266 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGGACATGGGTAGCAATT
Hairpin Sequence:
5'-CCGG-GAAAGGACATGGGTAGCAATT-CTCGAG-AATTGCTACCCATGTCCTTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGGACATGGGTAGCAATTCTCGAGAATTGCTACCCATGTCCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGGACATGGGTAGCAATTCTCGAGAATTGCTACCCATGTCCTTTC-3'

Other clones with same target sequence:

(none)