Construct: shRNA TRCN0000002906

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002849.x-1415s1c1
DNA Barcode:
TCAAGAGAGAAGAGGGTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRR (5801)
Gene Description:
protein tyrosine phosphatase receptor type R
Transcript:
RefSeq NM_002849.2 (NON-CURRENT)
Match location:
Position 1415 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002906)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGAGAGAAGAGGGTCCAA
Hairpin Sequence:
5'-CCGG-TCAAGAGAGAAGAGGGTCCAA-CTCGAG-TTGGACCCTCTTCTCTCTTGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGAGAGAAGAGGGTCCAACTCGAGTTGGACCCTCTTCTCTCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGAGAGAAGAGGGTCCAACTCGAGTTGGACCCTCTTCTCTCTTGA-3'

Other clones with same target sequence:

(none)