Construct: shRNA TRCN0000002959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003622.x-2559s1c1
DNA Barcode:
CCTCAATATAAGACCCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPFIBP1 (8496)
Gene Description:
PPFIA binding protein 1
Transcript:
RefSeq NM_003622.2 (NON-CURRENT)
Match location:
Position 2559 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAATATAAGACCCAGTTT
Hairpin Sequence:
5'-CCGG-CCTCAATATAAGACCCAGTTT-CTCGAG-AAACTGGGTCTTATATTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAATATAAGACCCAGTTTCTCGAGAAACTGGGTCTTATATTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAATATAAGACCCAGTTTCTCGAGAAACTGGGTCTTATATTGAGG-3'

Other clones with same target sequence:

(none)