Construct: shRNA TRCN0000002972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003660.x-3273s1c1
DNA Barcode:
GCTCGAATGTTAGATCACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPFIA3 (8541)
Gene Description:
PTPRF interacting protein alpha 3
Transcript:
RefSeq NM_003660.1 (NON-CURRENT)
Match location:
Position 3273 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGAATGTTAGATCACCTT
Hairpin Sequence:
5'-CCGG-GCTCGAATGTTAGATCACCTT-CTCGAG-AAGGTGATCTAACATTCGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGAATGTTAGATCACCTTCTCGAGAAGGTGATCTAACATTCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGAATGTTAGATCACCTTCTCGAGAAGGTGATCTAACATTCGAGC-3'

Other clones with same target sequence:

(none)