Construct: shRNA TRCN0000002974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003660.x-4694s1c1
DNA Barcode:
TCACGGGTAAAGAGAACTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPFIA3 (8541)
Gene Description:
PTPRF interacting protein alpha 3
Transcript:
RefSeq NM_003660.1 (NON-CURRENT)
Match location:
Position 4694 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGGGTAAAGAGAACTGTT
Hairpin Sequence:
5'-CCGG-TCACGGGTAAAGAGAACTGTT-CTCGAG-AACAGTTCTCTTTACCCGTGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGGGTAAAGAGAACTGTTCTCGAGAACAGTTCTCTTTACCCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGGGTAAAGAGAACTGTTCTCGAGAACAGTTCTCTTTACCCGTGA-3'

Other clones with same target sequence:

(none)