Construct: shRNA TRCN0000002978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003672.x-885s1c1
DNA Barcode:
TCTCACCATTCTCGACTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC14A (8556)
Gene Description:
cell division cycle 14A
Transcript:
RefSeq NM_003672.2 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000002978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCACCATTCTCGACTGTTT
Hairpin Sequence:
5'-CCGG-TCTCACCATTCTCGACTGTTT-CTCGAG-AAACAGTCGAGAATGGTGAGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCACCATTCTCGACTGTTTCTCGAGAAACAGTCGAGAATGGTGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCACCATTCTCGACTGTTTCTCGAGAAACAGTCGAGAATGGTGAGA-3'

Other clones with same target sequence:

(none)