Construct: shRNA TRCN0000003014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021090.x-3034s1c1
DNA Barcode:
CCTTGCCTTTAGCCGAATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTMR3 (8897)
Gene Description:
myotubularin related protein 3
Transcript:
RefSeq NM_021090.2 (NON-CURRENT)
Match location:
Position 3034 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCCTTTAGCCGAATGTA
Hairpin Sequence:
5'-CCGG-CCTTGCCTTTAGCCGAATGTA-CTCGAG-TACATTCGGCTAAAGGCAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCCTTTAGCCGAATGTACTCGAGTACATTCGGCTAAAGGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCCTTTAGCCGAATGTACTCGAGTACATTCGGCTAAAGGCAAGG-3'

Other clones with same target sequence:

(none)