Construct: shRNA TRCN0000003044

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014369.x-459s1c1
DNA Barcode:
GAGATAGAGAATGGGCGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN18 (26469)
Gene Description:
protein tyrosine phosphatase non-receptor type 18
Transcript:
RefSeq NM_014369.2 (NON-CURRENT)
Match location:
Position 459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003044)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATAGAGAATGGGCGGAAA
Hairpin Sequence:
5'-CCGG-GAGATAGAGAATGGGCGGAAA-CTCGAG-TTTCCGCCCATTCTCTATCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATAGAGAATGGGCGGAAACTCGAGTTTCCGCCCATTCTCTATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATAGAGAATGGGCGGAAACTCGAGTTTCCGCCCATTCTCTATCTC-3'

Other clones with same target sequence:

(none)