Construct: shRNA TRCN0000003140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015076.x-367s1c1
DNA Barcode:
GCTTGTAGAGAGATTGCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK19 (23097)
Gene Description:
cyclin dependent kinase 19
Transcript:
RefSeq NM_015076.1 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTAGAGAGATTGCACTT
Hairpin Sequence:
5'-CCGG-GCTTGTAGAGAGATTGCACTT-CTCGAG-AAGTGCAATCTCTCTACAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTAGAGAGATTGCACTTCTCGAGAAGTGCAATCTCTCTACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTAGAGAGATTGCACTTCTCGAGAAGTGCAATCTCTCTACAAGC-3'

Other clones with same target sequence:

  1. TRCN0000352638