Construct: shRNA TRCN0000003148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016203.x-1617s1c1
DNA Barcode:
GAAGTGCAATAAGCTGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAG2 (51422)
Gene Description:
protein kinase AMP-activated non-catalytic subunit gamma 2
Transcript:
RefSeq NM_016203.2 (NON-CURRENT)
Match location:
Position 1617 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGCAATAAGCTGGAAAT
Hairpin Sequence:
5'-CCGG-GAAGTGCAATAAGCTGGAAAT-CTCGAG-ATTTCCAGCTTATTGCACTTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGCAATAAGCTGGAAATCTCGAGATTTCCAGCTTATTGCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGCAATAAGCTGGAAATCTCGAGATTTCCAGCTTATTGCACTTC-3'

Other clones with same target sequence:

(none)