Construct: shRNA TRCN0000003180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004972.x-1578s1c1
DNA Barcode:
GCTTTGTCTTTCGTGTCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JAK2 (3717)
Gene Description:
Janus kinase 2
Transcript:
RefSeq NM_004972.2 (NON-CURRENT)
Match location:
Position 1578 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGTCTTTCGTGTCATTA
Hairpin Sequence:
5'-CCGG-GCTTTGTCTTTCGTGTCATTA-CTCGAG-TAATGACACGAAAGACAAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGTCTTTCGTGTCATTACTCGAGTAATGACACGAAAGACAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGTCTTTCGTGTCATTACTCGAGTAATGACACGAAAGACAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000315247