Construct: shRNA TRCN0000003295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000090.x-4132s1c1
DNA Barcode:
AGCTACGGCAATCCTGAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL3A1 (1281)
Gene Description:
collagen type III alpha 1 chain
Transcript:
RefSeq NM_000090.2 (NON-CURRENT)
Match location:
Position 4132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTACGGCAATCCTGAACTT
Hairpin Sequence:
5'-CCGG-AGCTACGGCAATCCTGAACTT-CTCGAG-AAGTTCAGGATTGCCGTAGCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTACGGCAATCCTGAACTTCTCGAGAAGTTCAGGATTGCCGTAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTACGGCAATCCTGAACTTCTCGAGAAGTTCAGGATTGCCGTAGCT-3'

Other clones with same target sequence:

(none)