Construct: shRNA TRCN0000003332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002006.x-755s1c1
DNA Barcode:
GTTACGGATGAGTGTTTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGF2 (2247)
Gene Description:
fibroblast growth factor 2
Transcript:
RefSeq NM_002006.2 (NON-CURRENT)
Match location:
Position 755 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTACGGATGAGTGTTTCTTT
Hairpin Sequence:
5'-CCGG-GTTACGGATGAGTGTTTCTTT-CTCGAG-AAAGAAACACTCATCCGTAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTACGGATGAGTGTTTCTTTCTCGAGAAAGAAACACTCATCCGTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTACGGATGAGTGTTTCTTTCTCGAGAAAGAAACACTCATCCGTAAC-3'

Other clones with same target sequence:

(none)