Construct: shRNA TRCN0000003416
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_020771.x-2606s1c1
- DNA Barcode:
- CCAGAAATTGATGTGAGTGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HACE1 (57531)
- Gene Description:
- HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
- Transcript:
- RefSeq NM_020771.2 (NON-CURRENT)
- Match location:
- Position 2606 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000003416)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGAAATTGATGTGAGTGAT
- Hairpin Sequence:
-
5'-CCGG-CCAGAAATTGATGTGAGTGAT-CTCGAG-ATCACTCACATCAATTTCTGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGAAATTGATGTGAGTGATCTCGAGATCACTCACATCAATTTCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGAAATTGATGTGAGTGATCTCGAGATCACTCACATCAATTTCTGG-3'
Other clones with same target sequence:
(none)
