Construct: shRNA TRCN0000003454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006267.x-9967s1c1
DNA Barcode:
CCTTGCATGGTGTGAAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RANBP2 (5903)
Gene Description:
RAN binding protein 2
Transcript:
RefSeq NM_006267.3 (NON-CURRENT)
Match location:
Position 9967 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCATGGTGTGAAATAAA
Hairpin Sequence:
5'-CCGG-CCTTGCATGGTGTGAAATAAA-CTCGAG-TTTATTTCACACCATGCAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCATGGTGTGAAATAAACTCGAGTTTATTTCACACCATGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCATGGTGTGAAATAAACTCGAGTTTATTTCACACCATGCAAGG-3'

Other clones with same target sequence:

(none)