Construct: shRNA TRCN0000003471

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020429.x-1431s1c1
DNA Barcode:
GCCCAGAGATACGAAAGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMURF1 (57154)
Gene Description:
SMAD specific E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_020429.1 (NON-CURRENT)
Match location:
Position 1431 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003471)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGAGATACGAAAGAGAT
Hairpin Sequence:
5'-CCGG-GCCCAGAGATACGAAAGAGAT-CTCGAG-ATCTCTTTCGTATCTCTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGAGATACGAAAGAGATCTCGAGATCTCTTTCGTATCTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGAGATACGAAAGAGATCTCGAGATCTCTTTCGTATCTCTGGGC-3'

Other clones with same target sequence:

(none)