Construct: shRNA TRCN0000003485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000062.x-924s1c1
DNA Barcode:
CCTCCTCAATGCTATCTACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPING1 (710)
Gene Description:
serpin family G member 1
Transcript:
RefSeq NM_000062.1 (NON-CURRENT)
Match location:
Position 924 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTCAATGCTATCTACCT
Hairpin Sequence:
5'-CCGG-CCTCCTCAATGCTATCTACCT-CTCGAG-AGGTAGATAGCATTGAGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTCAATGCTATCTACCTCTCGAGAGGTAGATAGCATTGAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTCAATGCTATCTACCTCTCGAGAGGTAGATAGCATTGAGGAGG-3'

Other clones with same target sequence:

(none)